resources

[this page is under construction]

prakash lab protocols

[coming soon!]

protocols for isolation and culture of primary glial cells

  1. Isolation and culture of microglia by Bohlen CJ, Bennett FC, and Bennett ML in Current protocols in immunology (2018).

  2. Isolation and culture of rodent microglia to promote a dynamic ramified morphology in serum-free medium by Collins HY and Bohlen CJ in the Journal of visualized experiments (2018).

  3. Purification and culture of astrocytes by Foo LC in Cold Spring Harbor protocols (2013).

biochem protocols

  1. Rapid, refined, and robust method for expression, purification, and characterization of recombinant human amyloid beta 1-42 by Prakash P, Lantz TC, et al (2019).

useful databases

  1. Brain RNA-seq - RNA-Seq of cell types isolated from mouse and human brain.

  2. GliaSeq - Repository of sequencing datasets generated by Liddelow lab and collaborators.

  3. Brain Cell Data - Mouse brain snRNA-seq cell and Slide-seq spatial transcriptomics atlas by Macosko Lab.